1 answer

You have a compound that has an absorption at 291 nm. At a concentration of 0.025...

Question:

You have a compound that has an absorption at 291 nm. At a concentration of 0.025 mM, the absorbance A291 = 0.40. What is its molar absorptivity of this sample (in M-1cm-1)? Assume you are using a 1 cm cuvette for your experiment.

Incorrect Question 1 0/2 pts You have a compound that has an absorption at 291 nm. At a concentration of 0.025 mM, the absorb

Incorrect Question 1 0/2 pts You have a compound that has an absorption at 291 nm. At a concentration of 0.025 mM, the absorbance A291 = 0.40. What is its molar absorbtivity of this sample (in M?cm-?)? Assume you are using a 1 cm cuvette for your experiment. 1,600

Answers

\epsilon = molar absorbtivity = absorbance / (concentration × path length)

Given, absorbance = 0.40, concentration = 0.025 mM = 0.025×10^-3 M and path length = 1cm

Thus \epsilon = 0.40 / (0.025×10^-3 × 1) = 16000 M^-1 cm^-1

Thus your answer to type is 16000.

.

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