# Yeast CDK (cdc2) has been fused with the mitotic cyclin (cdc13) and are together expressed from...

###### Question:

Yeast CDK (cdc2) has been fused with the mitotic cyclin (cdc13) and are together expressed from the cdc13 promoter (see figure below). Cells with this fusion go through the first cell stage but die in the next Gl. Explain hypothesize why cells with this fusion die in GI. If Cdc13-Cdc2 fused protein is injected in the cells described in "4" at the Gl from the second cell cycle they attempt to enter straight into M phase without passing through the intervening Sand G2 phases. Explain hypothesize why the rescue sends them straight into M-phase. Inject cdc13-cdc2 fused protein S M Arrest G1- cell cycle 1" cell cycle
1 2 3 Pcdc13 1 1446 1 L 894 cdc2cdc13 3' UTR Anti-Cdc13 cdc13 Anti-tubulin 1: Wild type 2: cdc 13-L-cdc2 A2A13 3: cdc13-L-cdc2 A2A13ACCP YFP Hoechst 20

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