1 answer

To prepare the protein sample, you need mix proteins and Laemmli buffer (5x) and then fuse...

Question:

To prepare the protein sample, you need mix proteins and Laemmli buffer (5x) and then fuse water to make up to 15ml . You have 4ml protein sample, calculate how many water and Lammeli Buffer you need to add? Laemmli final concentration should be 1x.


Answers

Final volume is 15 more and final concentration of buffer is 1x.

Initial concentration of buffer is 5x.

Using dilution law

MfVf = MiVi

Vi = 1x × 15/ 5x = 3 mL

Now you have to take 3 ml laemmli buffer, 4 ml protein sample and 15 - 3- 4 = 8 ml water for final concentration.

.

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