1 answer

See Hin Which of the following describes the Lacoperon in E.coli when lactose, but not glucose,...

Question:

See Hin Which of the following describes the Lacoperon in E.coli when lactose, but not glucose, is present in the culture med
See Hin Which of the following describes the Lacoperon in E.coli when lactose, but not glucose, is present in the culture medium? Choose one: A. The Lacrepressor, but not CAP, is bound to the Lacoperon's regulatory DNA and the Lacoperon is not expressed. B. Neither CAP nor the Lacrepressor is bound to the Lacoperon's regulatory DNA, and the Lacoperon is not expressed C. Neither CAP nor the Lacrepressor is bound to the Lacoperon's regulatory DNA, and the Lacoperon is expressed. D. CAP, but not the Lacrepressor, is bound to the Lacoperon's regulatory DNA, and the Lacoperon is expressed E. CAP and the acrepressor are both bound to the Lacoperon's regulatory DNA, and the Lacoperon is not expressed

Answers

D is the right answer.

Lac operon is a negatucely regulated and inducible operon.

The repressor is synthesised by the regulatory gene. In presence of lactose, lactose binds to the repressor. The repressor becomes inactive and ut can not bind to the regulator region i.e. operator in DNA. That's why RNA polymerase proceeds further to structural genes and their expression occur.

When glucose is absent, then cAMP binds to the CAP.

In this way CAP is in active form and binds to the regulatory DNA. This allows proper binding of RNA polymerase and structural gene expression occurs.

That's why right answer is D.

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