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**1** gram of agarose should be added to the solvent to make 2% agarose gel in 50 ml of solvent. We should you 2 gram of agarose in 100 ml of solvent to make 2% agarose gel. When the amount of the solvent becomes half then we should add half amount of agarose which is 1 gram. So, in the box **1** should be placed.

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