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first, amplify the gene using PCR, then clone the gene to the Ti plasmid, amplify the plasmid by transforming to bacteria, in the bacteria copy number of the plasmid increases, then transform Agrobacterium with the Ti plasmid, and infect the plants with the plasmid, then the plants with the plasmid can be selected using antibiotic.
so the order is 4,2,3,5,1,6
so the answer is c) 4,2,3,5,1,6.
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