Answers
We have 1U/ul and we need 0.1 U/ul for volume of 30 ul.
we use dilution formula M1V1 = M2V2 , 1 x V1 = 0.1 x 30
V1 = 3 ul ,
now for NEB , 10X x V1 = 4X x 30
V1 = 12 ul
Thus 3 ul of Fnu4HI stock solution and 12 ul of NEB buffer stock solution taken and then mixed and then diluted to 30 ml mark by water. Thus we get required solution.
.Similar Solved Questions
1 answer
I need help with this question. I need to calculate the volume amount of the reagent...
I need help with this question. I need to calculate the volume amount of the reagent needed which in my case is ammonium chloride ph: 8.75 . We need to prepare it as a 0.100 M aqueous solution to prepare a 70 ml sample of this buffer. Stations 13 to 16: Ammonium chloride buffer, pH 8.75 Stations ...
1 answer
（1） Wage discrimination occurs when people are paid different salaries based upon: education. working condition. experience....
（1） Wage discrimination occurs when people are paid different salaries based upon: education. working condition. experience. national origin. responsibility. (2) Table 32 The following table shows the distribution of income in Widgetapolis. Quintile Annual Family Inco...
1 answer
This is a two part question. A and B please it is one question only!!THANK YOU...
this is a two part question. A and B please it is one question only!!THANK YOU 5) Use the derivative of y = f(x) = x3 + 4x2 – x - 8 to a) determine the slope of the tangent line on the graph of y = f(x) at the point x = -3. b) give the equation of the tangent line to the graph of y = f(x) at x...
1 answer
Please answer with steps. a. Convert the following decimal numbers to 32-bit floating point number. 1....
please answer with steps. a. Convert the following decimal numbers to 32-bit floating point number. 1. 25.875 2.-7.0625 b. What decimal value is represented by the following 32-bit floating point number? 1. C10B000016 2. 46AB000016...
1 answer
When assessing D.m.'s injured ankle, what should be evaluated?
When assessing D.m.'s injured ankle, what should be evaluated?...
1 answer
6. A college library has five copies of a certain text on reserve. Two copies (1...
6. A college library has five copies of a certain text on reserve. Two copies (1 and 2) are first printings, and the other three (3, 4, and 5) are second printings. A student examines these books in random order, stopping only when a second printing has been selected. One possible outcome is 5, and ...
1 answer
6. Consider two coupled oscillators of mass mi and m2 that are attached by springs and are unstre...
6. Consider two coupled oscillators of mass mi and m2 that are attached by springs and are unstretched when x,-12-0. The damping force viscous dampers. The springs is proportional to the speed of the displacement and acts in the direction opposite the motion, Fd--cv T1 k1 k2 m1 m2 C1 C2 C3 a) Find t...
1 answer
10.4.37 Determine the upper-tail critical value of Fin each of the following one-tail tests for a...
10.4.37 Determine the upper-tail critical value of Fin each of the following one-tail tests for a claim that the variance of sample 1 is greater than the variance of sample 2. Click here to view page 1 of the table a. 0.01, n = 25, n - 11 Click here to view page 2 of the F table b. 0.025, n = 25, n ...
1 answer
Suppose the annual global supply and demand for oil (in billions of barrels) were QS =...
Suppose the annual global supply and demand for oil (in billions of barrels) were QS = -2+ 1P QD = 13 – 1 P Now suppose the technology for hydraulic fracturing is discovered, raising the quantity supplied by 2 billion barrels at any price. What is the new equilibrium quantity and price?...
1 answer
12. a-d and 13. a-d 12. Quality Air Conditioning manufactures three home air conditioners: an cconomy...
12. a-d and 13. a-d 12. Quality Air Conditioning manufactures three home air conditioners: an cconomy model a standard model, and a deluxe model. The profits per unit are S63-995, and $135, np tively. The production requirements per unit are as follows Number of Fans Number of Cooling Cods Manufa...
1 answer
(3) a) For the circuit shown in Figure Q3, calculate the voltage across the load RL....
(3) a) For the circuit shown in Figure Q3, calculate the voltage across the load RL. Assume the load is a welding system; calculate the voltage across the load considering the diodes are silicon (8 marks) b) Explain the role of the capacitor connected across the load. (2 marks) c) If the diodes D an...
1 answer
Please right the solution clearly. This question really important for me. THANKS! 12) In the P-V...
Please right the solution clearly. This question really important for me. THANKS! 12) In the P-V diagram shown in the figure, an ideal gas is processed to extract work. (R-8314 J/mol K, 1m3=1000L, 1atm=105Pa) The process from a to b is isothermal. a) Calculate work when isothermally gas is expanded...
1 answer
Who invented a vaccination for smallpox? Why was this vaccination so important?
Who invented a vaccination for smallpox? Why was this vaccination so important?...
1 answer
Question 15 While preparing the bank reconciation for Calvinsch unter the 3 korte tuttethette og loss...
Question 15 While preparing the bank reconciation for Calvinsch unter the 3 korte tuttethette og loss 579741 2 500 Calvin's balance stording to the business hout 154 Deposits in transit 3.5 Bark service charge A customer's non-sufficient funds NSP cheque reund by the bank What amount wil Cal...
1 answer
A cylindrical tube has diameter 12 cm and length 22 cm. What is the curved surface area of the tube, to the nearest square centimeter?
A cylindrical tube has diameter 12 cm and length 22 cm. What is the curved surface area of the tube, to the nearest square centimeter?...