Answers
1. Pure culture is the type culture used in this streak plate.
2. Bacteria in pure culture shows different response compared to mixed culture
in mixed culture one species of bacteria may facilitate or weaken the response
of other bacteria so microbiologist can use pure culture to specifically study about a
microorganism.
3.* Pure culture can obtained in streak plate method by streaking the mixed culture across the
surface of a agar medium with the help of a loop and during streaking the inoculum appropriately
result in seperation of microorganisms from each other.
* After streaking out to a second area of plate using the same loop incubation should be done for
allow the growth of bacterial colonies.
* In above case can obtain a pure culture due to use of streak plate method for isolating single colony
of bacteria and it can observe with the help of a microscope.
4. After doing streak plate of a specimen incubate the plate for 24 to 48 hours at a temperature
of 37 degree celcious to obtain isolated colonies.
5.Three reasons to use sterile technique in microbiology laboratory
* Use of sterile techniques help to avoid spread of unwanted contaminated
microorganisms in the laboratory.
* It prevent transfer of infections to microbiologist who work in the loboratory.
* It directly influence accuracy of lab results about different type of infections.
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