# INSTRUCTIONS You may print out this assignment and fill it in by hand. We suggest using...

###### Question:

INSTRUCTIONS You may print out this assignment and fill it in by hand. We suggest using pencil in case you make mistakes!! Submit your Assignment as a single doc on Canvas. ASSIGNMENT 1) For the DNA sequence given below, write the complementary DNA sequence that would complete the double-strand. DNA 3-A TTGCT TACTTGCA T-5° DNA 5 2) Does it matter which strand is the 'code strand'? The following two sequences look identical, except one runs 3-5' and the other 5'-3'. For each DNA sequence given below, write the mRNA sequence that would be coded from it. Make sure you indicate the direction of each mRNA strand (i.e. 3' and 5'ends). Use the Universal triplet code to determine the sequence of amino acids that would be generated for each of the mRNA sequences that you generated in question 2. Remember that the reading of mRNA goes in the 5'-3' direction (see lab notes for examples). . To make the mRNA, read from LEFT TO RIGHT- To make the amino acid, read triplets from Left to Right DNA 3-TACCTACTTTGCCCGATCCAT-5' mRNA 5... amino acid b. Read letter by letter from RIGHT TO LEFT To make the amino acid, read triplets from Left to Right DNA 5'-TACCTACTTTGCCCGATCCAT-3 mRNA 5. amino acid c. Are the amino acid sequences in a and b the same? YES_
3) What 3 to 5 DNA sequence would correspond to a s AUG.3-start codon? U 6–3' RNA 5-A DNA 3'- 4) Read the DNA sequence given below in the 3 to 5' direction and circle all of the potential start codons (i.e. every time you see the DNA version of a start codon- as in qu 3). How many potential start codons did you find? Line YOUR DNA SEQUENCE (read from left to right) 3- TTCCGTAACTTCGGCGCATCTAGCTTGAGCTCCAATCAGG ACTACTTATAAA AATCTTCTCGAGAGAGCTTTACTCCTACTC TCTTAGTCGATTCCATCGGACCTACGATTGACAAGCGCGGTC TACTATCTACTTATTTATTTACGAGCGTTGATTCTACCTACTG ACGACTAGGGCATICTATAGGATTAACTCCITATTTTAACTA ACTTCCTGGGAAGGCGCCTTTATC TGATCCGTAATCCGTCCG AAGGCTCTGATCUGATTACTGGGTCACTGGAAAGTGACCGCT GTCTATTACTGTATTTCATCTGATTGACTATTITATAGTCG-5' 5) Now find and circle the promoter region 3'-TATAAAA-S' in your sequence above,
6) Identify the start codon that is immediately downstream (towards the 5' end, after the promoter) of the promoter. Indicate the codons that follow the start, and continue until you reach a stop codon. 7) Copy the entire coding sequence that is found in question 4 (from, and including the start codon to the stop codon). Hint: there are 19 codons, each with 3 base pairs, and the coding region is between lines 2 and 4. 5 6 8)Now write down the mRNA sequence that would be generated from the DNA sequence from question 7. Hint: there are 19 codons, each with 3 base pairs. 9) Finally write out the sequence of amino acids that the mRNA sequence in question 8 would code for. Hint: there are 18 amino acids (including the start/methionine- remember that STOP codon does not code for an amino acid). Use the Universal Triplet code from the Gene Expression tutorial document, ..... ... .. 10 11 12 13 14 15 16