###### Question:

1. Answer the following questions about chromosomal and extra-chromosomal DNA: A. A previously undiscovered single-cell organism was found living at a great depth on the ocean floor. Its nucleus contained only a single linear chromosome with 7* 106 nucleotide pairs of DNA coalesced with three types of histone-like proteins. A short micrococcal nuclease digestion yielded DNA fractions of 700, 1400, and 2100 bp. Predict what these fractions represent. What conclusions can be drawn? B. After salivary gland cells from Drosophila are isolated and cultured in the presence of radioactive thymidylic acid, autoradiography is performed, revealing the presence of thymidine within polytene chromosomes. Predict the distribution of the grains along the chromosomes.

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