# A1 is my DNA, Enzyme 1 BsrB1 P1 is known pBR322 enzyme 1 A2 is my...

###### Question:

A1 is my DNA, Enzyme 1 BsrB1
P1 is known pBR322 enzyme 1
A2 is my dna enzyme 2 RSa1
p2 is known pBR322 enzyme 2
Au is my dna uncut
Pu is know pBR322 uncut
ladder Pu Au P, A, P2 Az HAAAM .. - וור
RESULTS: Analyzing your data Tobbul Avdinol Answer the following questions: 1) How many bands do you see in the "uncut" lanes? If there are more than one band can you explain the nature or origin of the other bands? 2) Why did you have to clectrophorese uncut DNA? 3) What size of fragments were you expecting when you digested the known samples of PBR322 with the enzymes you used? 4) What are the sizes of the bands that you actually see? 5) Are there any other anomalous bands in these lanes? If so, can you explain how these may have arisen? 6) Does the plasmid you isolated correspond to pBR322? Use the photograph of your results to argue one way or the other. This is the central question, which should help support or negate our original hypothesis
Clone Sizer DNA Ladder 2686 2000 1500 1200 וווווווווון saa888 400 300 200 100dboonstored bobo

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