1 answer

5) Ion exchange chromatography is based on the A) electrostatic attraction B) electrical mobility of ionic...

Question:

5) Ion exchange chromatography is based on the A) electrostatic attraction B) electrical mobility of ionic species C) adsorpt
5) Ion exchange chromatography is based on the A) electrostatic attraction B) electrical mobility of ionic species C) adsorption chromatography D) partition chromatography

Answers

Ion exchange chromatography is based on the (A) electrostatic attraction.

The principle of this chromatography is that electrostatic attraction binds charged molecules (say negatively charged protein) with the oppositely charged stationary phase (eg. positively charged matrix).

Following the binding event, ionic strength is altered to exchange the bound molecules with ions having a similar charge.Positively charged protein binds to negatively charged bead Large partire charge Negative che Powe Negatively charged protein

.

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