1 answer

32. Work this dilution problem, solve for the number of colony forming units (cfu) in one...

Question:

32. Work this dilution problem, solve for the number of colony forming units (cfu) in one mL of the E. coli sample. ml 1 ml.
32. Work this dilution problem, solve for the number of colony forming units (cfu) in one mL of the E. coli sample. ml 1 ml. 1ml. 1ml. 1m. 1 ml A 2 a. 6.3 x 10 b. 6.3 x 10 c. 4.43 x 106 9 ml. 9 ml. d. 4.43 x 103 9mL Sample of E. coll 443 63 colonies colonles

Answers

32) The answer will be 6.3 x 106 (Option a).

Explanation: The countable range for colony is 25-250 CFU/plate. Thus, during calculation we will consider the plate that contains 63 colonies.

Now, dilution factor = Volume of stock solution taken/ Final volume

Now, 1 mL of stock solution is taken from flask to the first tube which contains 9 mL of diluent.

So, dilution factor of first tube = 1/ (1+9) = 1/10 = 10-1 dilution

Similarly, in each case there will be 10-1 dilution as similar process is followed in each case.

So, in fifth tube (From which 63 colonies grown on the plate) total dilution factor will be 10-5.

Now, concentration of fifth tube is 63 CFU/mL (As 63 colonies are grown on the plate by taking 1 mL. Please note that there is no mention that 1 mL or other values of solution taken from this tube for plating. We assume 1 mL & this is only possible from the given answer options).

Now, cell concentration in fifth tube = Initial cell concentration in flask x total dilution factor (TDF) of fifth tube

So, Initial cell concentration in flask = Cell concentration in fifth tube / Total dilution factor (TDF) of fifth tube = (63 / 10-5) CFU/mL = 63 x 105 CFU/mL = 6.3 x 106 CFU/mL

.

Similar Solved Questions

1 answer
5. 0/1.53 points | Previous Answers SerCP10 15.P049 My Notes Ask Your Teacher In deep space...
5. 0/1.53 points | Previous Answers SerCP10 15.P049 My Notes Ask Your Teacher In deep space two spheres each of radius 4.80 m are connected by a 1.80 x 102 m nonconducting cord. If a uniformly distributed charge of 37.0 mC resides on the surface of each sphere, calculate the tension in the cord 115 ...
1 answer
Use the component method to add the vectors A and B shown in the figure. Both...
Use the component method to add the vectors A and B shown in the figure. Both vectors have magnitudes of 2.95 M and Vector A makes an angle of theta = 25.0 degree with the X A+B= D Web Page 3.P022 Use th axis. Express the resultant A + B in unit-vector notatio A+B e component method to add the vect...
1 answer
Research question- will treating alzheimers early onset before 30years help the prevention of alzheimers later on??...
research question- will treating alzheimers early onset before 30years help the prevention of alzheimers later on?? why? how? or why not? statistics would help too!!...
1 answer
Aye/5D2 TOUTE Problem Statement Write a program, using pseudocode, that asks the user for two numbers....
aye/5D2 TOUTE Problem Statement Write a program, using pseudocode, that asks the user for two numbers. Your program should determine the relationship of the numbers using = <or>. You should output one of the following results: NOTE: Your program should use nested IF-THEN-ELSE statements. Do no...
1 answer
Part 1: A mobile phone service provider has three different data plans for its customers: Package...
Part 1: A mobile phone service provider has three different data plans for its customers: Package A: For $39.99 per month 4 gigabytes are provided. Additional data costs $10 per gigabyte. Package B: For $59.99 per month, 8 gigabytes are provided. Additional data costs $5 per gigabyte. Package C: For...
2 answers
What will be the integration path used in Ampere's law to calculate the magnetic field B?...
What will be the integration path used in Ampere's law to calculate the magnetic field B? at a distance r from the axis of the inner cylinder? A solid cylindrical conductor is supported by insulating disks on the axis of a conducting tube with outer radius Ra = 6.15cm and inner radius Rb = 3.3...
1 answer
10) Respiration in the breathing sense refers to an exchange of gases. How is your breathing...
10) Respiration in the breathing sense refers to an exchange of gases. How is your breathing related to your cellular respiration? provide the overal chemical equation for cellular respiration and compare the rectants, and products, and energy yield of the three stages of cellular respiration....
1 answer
Two-lens systems. In the figure, stick figure (the object) stands on the common central axis of...
Two-lens systems. In the figure, stick figure (the object) stands on the common central axis of two thin, symmetric lenses, which are mounted in the boxed regions. Lens 1 is mounted within the boxed region closer to O, which is at object distance p1. Lens 2 is mounted within the farther boxed region...
1 answer
On May 28, 2021, Pesky Corporation acquired all of the outstanding common stock of Harman, Inc.,...
On May 28, 2021, Pesky Corporation acquired all of the outstanding common stock of Harman, Inc., for $590 million. The fair value of Harman's identifiable tangible and intangible assets totaled $631 million, and the fair value of liabilities assumed by Pesky was $169 million. Pesky performed a g...
1 answer
Expand 2 cos(24u) using a double angle identity in terms of sines.
Expand 2 cos(24u) using a double angle identity in terms of sines....
1 answer
How do you find the slope of a line perpendicular to #2x+3y=5#?
How do you find the slope of a line perpendicular to #2x+3y=5#?...
1 answer
According to the text, compared with larger societies-particularly modern societies, such as the United States-most hunting...
According to the text, compared with larger societies-particularly modern societies, such as the United States-most hunting and gathering groups were: egalitarian brutish nasty authoritarian...
1 answer
An experiment has a sinale factor with 3 aroups and 5 values in each aroup. In...
An experiment has a sinale factor with 3 aroups and 5 values in each aroup. In determining the among-group variation, there are 2 degrees of freedom. In determining the within-group variation, there are12 degrees of freedom In determining the total variation, there are14degrees of freedom. Also, not...
3 answers
Count the number of transitions
Design the minimum-state DFA that accepts all andonly the strings of 0's and 1's that have 110 as a substring. Toverify that you have designed thecorrect automaton, we will askyou to identify the true statement in a list of choices. Thesechoices will involve:The number of loops (transitionsf...
1 answer
A library crew can shelve books in six hours
A library crew can shelve books in six hours. Another crew can do the job in four hours. If the first crew begins and is joined by the second crew one hour later, how many hours will it take to finish the job?...
1 answer
Referring to the Interactive Figure below, suppose two astronauts are using jet packs to push a...
Referring to the Interactive Figure below, suppose two astronauts are using jet packs to push a 940 kg satellite toward the space shuttle, as shown in the figure below. With the coordinate system indicated in the figure, astronaut #1 pushes in the positive x-direction and astronaut #2 pushes in a di...
1 answer
E1-19 E1-19 Using accounting vocabulary Consider the following accounting terms and definitions, and match each term...
e1-19 E1-19 Using accounting vocabulary Consider the following accounting terms and definitions, and match each term to the definition: Learnin 1. Accounting equation a. An economic resource that is expected to be of benefit in the future 2. Asset b. Debts that are owed to creditors 3. Balance shee...
1 answer
23. The pH of 250 ml of 0.2 M aspartic acid is 2.2. Sketch the titration...
23. The pH of 250 ml of 0.2 M aspartic acid is 2.2. Sketch the titration curve obtained by titrating the aspartic acid solution with 0.5 M KOH show clearly how you obtain the values used to plot the curve. (pKa1 = 2.0 , pKa2 = 3.8 , pKa3 =9.8)...