# 3. Rapid cell responses are often generated by positive feedback actinomcin D loops. An experiment was...

###### Question:

3. Rapid cell responses are often generated by positive feedback actinomcin D loops. An experiment was done to test if the rapid release ofUV light cytochrome c from mitochondria in response to cell damage results from this type of feedback loop (ex: caspases activated bv released cvtochrome c ca act on mitochondria to release more cytochrome c). Cells expressing a GFP-tagged cytochrome c were incubated in the presence or absence of a caspase inhibitor called yZAD and then exposed to different apoptosis-inducing agents. The time it took for cytochrome c to be released during each treatment was measured and the results are shown in the figure. 10 ZVAD Figure 18-5 Duration of cytochrome c- GFP release from mitochondria in cells What would you have expected the results to look like if a treated with various apoptosis-inducing positive feedback loop between caspase and cytochrome c agents in the presence and absence ofa release was involved? Do the actual results match this caspase inhibitor (Problem 18-21). Bars expectation? represent the average of measurements on multiple individual cells

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