# 11. What substance did we use as negative control in our ELISA experiment? (2 pts) 12....

###### Question:

11. What substance did we use as negative control in our ELISA experiment? (2 pts) 12. We rinsed the wells with twice with PBS-Tween and the only once with PBS afterwards. What is the purpose of rinsing with PBS after PBS-Tween? (5 pts) 13. Name one infectious disease that is usually diagnosed by an Indirect ELISA method: (3 pts) 14. What substance did we used as a blocking agent in the ELISA experiment? (5 pts) A. PBS-Tween B. Biotin C. Streptavidin-peroxidase D. BSA E. TMB 15. We put "blocking reagent" after coating the wells with antigen. What is the purpose of that? Explain what may happen if you don't put the blocking reagent in. (5 pts) 16. During electrophoresis, the gas that comes out at the positive end is: (3 pts)

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