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1. During both DNA replication and PCR the DNA double helix must be unwound, in DNA replication process DNA strand are unwound by enzyme called helicase which break hydrogen bonds between two strands, and in PCR it is achieved by high temperature at 94 degree C.
2. A methylguanosine cap and poly A tail are added to mRNA molecule after transcription, these modification of mRNA is important because it protect mRNA from enzymatic degradation in cytoplasm also help to bind mRNA to translation complex.
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