Which ofthe following is a palindromic restriction site?
A. 5' - ACGGCA - 3'
B. 5' - TGCGCC - 3'
C. 5' - AAGGTT - 3'
D. 5' - ACGCGT - 3'
E. 5' - GCAGCA - 3'

2 answers

Question:

Which ofthe following is a palindromic restriction site?
A. 5' - ACGGCA - 3'
B. 5' - TGCGCC - 3'
C. 5' - AAGGTT - 3'
D. 5' - ACGCGT - 3'
E. 5' - GCAGCA - 3'

The palindromic restriction site can be seen in Option D. 5' - ACGCGT - 3' from the following given options in the question.A palindromic restriction site occurs in restriction enzymes that cut the double-stranded DNA of the enzyme at specific locations. They do so when recognized that the sequence is palindromicThe palindromic sequence is a little series of base pairs that follow a set of ordered rules.We know that;Adenine A = Thymine TGuanine G = Cytosine CSo, we will pick the first three pairs of the given bases and complement them in reverse order.Then, reverse the order of the complement and put it in front of the end of the forward strand.Starting with Option A 5' - ACGGCA - 3'ACG → TGCthe reverse of the complement = CGTadding it to the forward strand, we have 5' - ACGCGT - 3' which is not equal to the given option.Option B5' - TGCGCC - 3'TGC → ACGthe reverse of the complement = GCAadding it to the forward strand, we have 5' - TGCGCA - 3' which is not equal to the given option. Option C5' - AAGGTT - 3'AAG → TTCthe reverse of the complement = CTTadding it to the forward strand, we have 5' - AAGCTT - 3' which is not equal to the given option.Option D5' - ACGCGT - 3'ACG → TGCreverse of the complement = CGTadding it to the forward strand, we have 5' - ACGCGT - 3' which is equal to the given option.Option E5' - GCAGCA - 3'GCA → CGTthe reverse of the complement = TGCadding it to the forward strand, we have 5' - GCATGC - 3' which is not equal to the given option.Therefore, we can conclude that option D is the only correct palindromic restriction site.Learn more about the palindromic restriction site here:https://brainly.com/question/8886948?referrer=searchResults

answer from ajeigbeibraheem

3

Answers #2

Answer:which off the following is a paliindromic restriction site E GCAGCA-3

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